Master Thesis

Get the odor: Characterization of improved CAR variants

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Kontakt: Dr. Margit Winkler
Email: Nicht verfügbar
Telefon: 0316-873 9333

Ref. No. 04_2018


The Austrian Centre of Industrial Biotechnology (acib) is bridging the gap between science and industry and develops more environmentally friendly and economic processes for the biotechnological, pharmaceutical and chemical industries. All these processes are modeled on methods and tools from nature. This know-how is the foundation for new and improved applications and products in the field of industrial biotechnology all over the world. Currently, more than 250 acib scientists are working on more than 150 industrial and strategic projects. In Graz we currently offer a


Master thesis


Get the odor: Characterization of improved CAR variants


More than probably any other sense, smell evokes memories and aldehydes are the key ingredients for creating these moments. Bioreduction of carboxylic acids to aldehydes by Carboxylic Acid Reductases (CARs) show promising results for a more sustainable route to aldehydes. CARs are, for example, industrially used for vanillin production. Just recently, structural data of these multi-domain enzymes was published. Our research group combines extensive knowledge for generating improved enzyme variants.

In this project, we are specifically interested in investigating the characteristics of these enzyme variants. Therefore, cloning and heterologous expression will be the starting point to allow enzyme purification and characterization. Investigation of the substrate scope in vivo and in vitro will enable to assess the potential of these new enzymes for biotechnological application.

The ideal master candidate studied molecular biology, biochemistry or biotechnology and has very good theoretical and practical knowledge of: molecular biology methods (PCR, DNA extraction and purification, gel electrophoresis, cloning); bioinformatic tools (BLAST, CLC-workbench); microbiological work such (bacteria cultivation on small scale, cell disruption, protein purification, NuPAGE analysis and enzyme activity assays with HPLC and GC analysis). However, knowledge of all techniques is not mandatory. Applicants should be enthusiastic to reach defined goals, to be motivated to learn new techniques and to present their results.

We, the team of Dr. Margit Winkler, are working in an international environment and are looking for motivated candidates with good English skills both spoken and written. Starting dates are flexible.

Deadline for applications: March 19th 2018

If you feel addressed or would like further information, please contact Dr. Margit Winkler ( or come by at Petersgasse 14, 3rd floor, 8010 Graz.


ACIB is dedicated to a well balanced gender ratio. Therefore this job advertisement addresses qualified male and female persons equally. ACIB is not part of any collective agreement under Austrian law.



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